A. The Compound Microscope
5.1 How does the image move in the field of view, in relation to the movement of the slide?
5.2 From these observations, what can you deduce about the path of light in a body tube?
Light is refracted
6.1 Is the position of the object in the field changed by shifting from LPO to HPO? Why?
6.2 Is there a decrease or an increase in the area of the specimen seen in the field of vision when changing from LPO to HPO? Why?
Yes. Increase in magnification.
Table 1.1 Parts and Functions of a Compound Microscope
Ocular lens / Eyepiece – lens located at the upper end of body tube to look through
Tube – connects eyepiece to objective lens
Arm – supports tube and connects to base
Stage – platform where the slide is placed
Stage clips – used to hold the slide in place
Revolving nosepiece / turret – holds 2 or more objective lenses and can be rotated to easily change objective power
Objective lenses - used for magnification along with the eyepiece. LPO/HPO/Oil immersion
Dust tube – protects objective power
Course adjustment knob – used to raise or lower objective power
Fine adjustment knob – used for critical focusing
Base – bottom of microscope. Used for support
Diaphragm – controls the amount of light that will enter the microscope
Mirror – used to reflect light from an external light source up through the bottom of the stage
Mirror rack – holds mirror in place
Inclination joint – used to incline the microscope
B. The Dissecting Microscope
8.1 How is the movement of the slide related to that of the image? Why?
Moves in same direction. Single lens.
Note: The next few questions are dependent on the microscope/specimen used and is therefore different for each student. You’ll have to actually do the experiment, sorry.